Reintroduction of a female Black rhino in its natural habitat, in South Africa. Since then, this female has already contributed with at least five new cubs, which are crucial to the survival of this species, classified as critically endangered.

The Lisbon Zoo has supported the program of in situ conservation of okapis (Okapia johnstoni) since 2006, in the Democratic Republic of Congo. It aims to: train and equip the guards of the Okapi Wildlife Reserve, in the Ituri forest; take action in the environmental education of local populations; and collaborate with the Center for Training and Research in Forest Conservation, in Epulu, Ituri. 

The aim of the Cameroon Wildlife Aid Fund (CWAF) is to protect large primates in their natural habitats. To that effect, it created the Mefou National Park in Cameroon, managed in partnership with local government authorities. The Zoo has supported this program of in situ conservation in Cameroon since 2006.

The Addax has already been extinct in the wild. However, this species has been recovering and is being reintroduced into its natural habitat. The Lisbon Zoo has participated by sending a couple of addaxes in a project in which 70 animals from 16 different zoos were reintroduced in the National park of Souss-Massa, in Morocco. And later, in 2005, the Lisbon Zoo sent more addaxes for reintroduction, this time to Tunisia, through the respective EEP. 

The Lisbon Zoo participates in the Scimitar-horned oryx EEP, and has been financially supporting their in situ conservation. In 2007, a new herd of this species was established in the Natural Park of Dghoumes (Tunisia), through the translocation of animals and the reintroduction, in late 2007, of animals from the EEP and the SSP.

The Lisbon Zoo collaborates with the in situ conservation program of the Grévy zebra, in Ethiopia and in Kenya. This program is coordinated by the Grevy's Zebra Trust, to meet the urgent need to conserve this species in these countries. A noteworthy fact is that in 2012, this project received the International Conservation Award from the American Association of Zoos and Aquariums (AZA).

Through The Conservation Fund, the Lisbon Zoo participates in emergency actions with essential financial contributions. As an example, one could highlight the rescue of African penguins from an oil spill near Cape Town, South Africa.

This island is home to species that are not found anywhere else in the world, such as lemurs and a great variety of chameleons and amphibians. Given that only five to eight per cent of its original tropical forest remains, the endemic species of Madagascar are threatened. In 2007, together with the Zoo of Doué-la-Fontaine, in France, and the Antongil Conservation, the Lisbon Zoo launched an important conservation program focused on one of the last intact forests of Madagascar: the Farankaraina Forest. This program has ensured the monitoring of the forest’s fauna and flora since 2008, and also acts in the environmental education of the local population.